Emblica officinalis is the very popular medicine in Ayurveda, used since ancient time as a medicine for the treatment of various diseases. In the present study we have isolated the pharmacologically active marker constituents from amla and its identification was done by TLC and RP-HPLC methods. The amla wine was prepared by using Saccharomyces cerevisiae as a fermenter with addition of jaggery. The self generated alcohol (SGA) content as ethanol was observed to be 9.29%, also the higher alcohol like n-butanol, n-propanol, iso-butanol were also found in trace amount as a byproduct of alcoholic fermentation. Heavy metal analysis was carried out using AAS. The UV spectrophotometric method for amla wine was developed and validated as per ICH Q2B guidelines.
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